ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism

Here, we demonstrate the first steps of data analysis for some typical ELISA data. The procedure shown is as follows: 1) Compare the arrangement of our samples on the 96-well plate with the absorbance measurements obtained from the plate reader. 2) Subtract out the blank measurements from all the entire plate. 3) Arrange the absorbance measurements of the standards next to their corresponding known concentrations. 4) Place the absorbance measurements of the samples below the absorbance measurements of the standards.

In the next video of this series, we will demonstrate how to interpolate the concentration of our samples using software called GraphPad Prism. Click here for a free 30-day trial of GraphPad Prism – http://protocol-place.com/Prism

This video and other protocols can be found at our website, the “Protocol Place” – http://protocol-place.com/

A full ELISA protocol can be found at http://protocol-place.com/assays/sandwich-elisa-protocol/

This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series:

ELISA Tutorial 1: Understand How an ELISA Works – http://youtu.be/nNjlBCnpGZ4

ELISA Tutorial 2: Coating and Blocking the ELISA Plate – http://youtu.be/AmG7FBolfdc

ELISA Tutorial 3: Preparing and Adding Samples to the ELISA Plate – http://youtu.be/darrx6F0wsg

ELISA Tutorial 4: Finishing the Assay (Sandwich ELISA) – http://youtu.be/zI4khIJhCd8

ELISA Tutorial 5: Preparing ELISA Data in Excel for Analysis with GraphPad Prism – http://youtu.be/l9tO81ZCeRg

ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism – http://youtu.be/5IqqpKSnXfI

Competitive ELISA Tutorial 1: How a Competitive ELISA Works – http://youtu.be/Kb26nQVMHds

Competitive ELISA Tutorial 2: How to Use Calbiotech’s Competitive ELISA Kits – http://youtu.be/3E_U_4Z2dUc

Competitive ELISA Tutorial 3: Analyzing Typical ELISA Data in Excel – http://youtu.be/s2t0jiWxiDI

We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to “like,” or better yet, share them with others!

Thanks for watching!

Youssef Farhat

Protocol Place: http://protocol-place.com

***Check out some of our other tutorials via the links below***

Competitive ELISA Tutorials – – – http://www.youtube.com/watch?v=Kb26nQVMHds&list=PLR4wfoQ4HbymisBtft-i-QTsyRDMFymC3

ELISA Tutorials – – – http://www.youtube.com/watch?v=nNjlBCnpGZ4&list=PLR4wfoQ4HbynbS01zeuBV-awsOAxDPhYO

Gelatin Zymography Tutorials – – – http://www.youtube.com/watch?v=MF2sWQSaBWg&list=PLR4wfoQ4Hbykrj7rxk6i5jzkjtvTtZUVx

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